Description: TJUSLS China's subject of the competition for this year is the surface display to modify PET hydrolase(PETase). PET hydrolase was found from a kind of microorganism living on PET as the main carbon source. It can degrade macromolecular polymers into monomers. Surface display can reveal the protein whose gene code is coalescing the gene code of target protein or polypeptide with the counterpart of ankyrin on the surface of the host cell wall to harvest the whole cell catalyst. The protagonists of our project, which are PETase and the surface display technology, will act in two aspects. Firstly, create the mutant of PETase in order to improve the degradation efficiency and thermal stability. Secondly, useing surface display on the surface of the prokaryotic (Escherichia coli) and eukaryotic (Pichia yeast) for whole cell enzyme catalysis reaction.
Collaboration details:
Year: 2016Visit Wiki
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Updated at: 8/9/16