Bilkent-UNAMBG

Description: Cancer is one of the most common and lethal disease in the world and it is always highlighted that early diagnosis is crucial in terms of cancer treatment. It is often invasive and painful to diagnose cancer with the existing methods. At this point, breath test appears as a promising non-invasive and real-time technique which allows the monitoring of metabolic status. Volatile organic compounds(VOCs) in the exhaled breath provide in vitro detection, classification and discrimination of diseases and microorganisms. Concentration of specific VOCs in the exhaled breath can either increase or decrease depending on the type of diseases and microorganisms. In this project, our goal is to produce a prototype which can detect and discriminate four most common cancer types: lung, breast, colorectal and prostate. In order to achieve this, we will engineer an artificial plasmid including promoter and reporter genes that are specific to a particular VOC. It is expected that when that particular VOC is given into the environment, bacteria will express reporter gene hence we will get some signal. After achieving getting the signal for a particular VOC, we aim to construct logical gates and circuits by using CRISPRi system on plasmids to create well accoutred bacteria which will be able to differentiate these four cancer types. Our future goal is to transform our prototype into a kit. So that, diagnosis of cancer will be simpler and non-invasive.
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Year: 2016Visit Wiki
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Updated at: 8/9/16